The sample preparation workflow for targeted sequencing requires an additional step of target enrichment. Allows more samples to be analyzed than whole genome sequencing.Offers a more manageable dataset for subsequent bioinformatics analysis.Provides cost effectiveness, time and resource savings, and precision.Enables higher coverage, deeper sequencing and straightforward data analysis.Allows detection and quantification of rare and low-frequency variants.For example, targeted resequencing of the polymorphic human leucocyte antigen (HLA) gene helps in HLA typing, which is crucial for matching in hematopoietic stem cell or solid organ transplantation. The focused approach of targeted sequencing provides the possibility of its use in targeted therapy applications and in personalized medicine efforts. Targeted sequencing of specific regions also enables the discovery of causative genes for rare diseases. Because of this sensitivity, targeted sequencing provides tremendous advantage in variant calling in cancer research, identification of disease-associated mutations, single gene disorders and in gene expression studies. It also enables deeper analysis of results than WGS and other survey approaches. In addition, it allows for deeper sequencing, and the depth of coverage helps in avoiding false interpretations of sequencing data. Targeted sequencing is more cost-effective than whole genome sequencing (WGS). This application allows targeting of specific genes, coding regions, even segments of chromosomes with precision and efficiency. It is a powerful application for investigating a variety of disease areas, such as oncology, inherited diseases, immunology and infectious diseases. Targeted sequencing offers unique insights into specific regions of interest in the genome. Focus your sequencing efforts on the genomic regions of interest
0 kommentar(er)
